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Department of Plant Biotechnology
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Plant x Environment Interaction

Plant x Environment Interaction

Department of Plant Biotechnology

How plants respond to a changing environment

We investiagte plant responses to changes in the abiotic environments in order to understand how acclimation is brought about. Low temperatures or light intensity are a focus of our work. We use the model plant Arabidopsis thaliana that is native to Europe, where it builds various local populations that are adapted to their specific environmental conditions.

Low temperature, water deficit or soil salinity have in common that they cause dehydration of the cells. This causes solutes to accumulate, and in extreme cases can stall metabolism. Even more important is the danger of membrane damage: organellar membranes and the plasma membrane loose fluidity, when hydration of the lipid head groups is lost. Membrane rupture or membrane fusions can cause cell death.

During acclimation to low temperatures, soluble sugars are accumulated that can protect membranes and cellular proteins. We could show that individual sugars act differently in differen subcellular compartments (Knaupp et al., 2011). Distribution of sugars within the cell changes during cold acclimation (Nägele & Heyer, 2013). Mathematical simulations show that the extend of these changes is correlated with freezing tolerance.

Related Publications

Hoermiller, I.I., Funck, D., Schönewolf, L., May, H., Heyer, A.G.: Cytosolic proline is required for basal freezing tolerance in Arabidopsis. Plant, Cell & Environment. (2022). https://doi.org/10.1111/pce.14196.
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@article{https://doi.org/10.1111/pce.14196, abstract = {Abstract The amino acid proline accumulates in many plant species under abiotic stress conditions, and various protective functions have been proposed. During cold stress, however, proline content in Arabidopsis thaliana does not correlate with freezing tolerance. Freezing sensitivity of a starchless plastidic phosphoglucomutase mutant (pgm) indicated that localization of proline in the cytosol might stabilize the plasma membrane during freeze–thaw events. Here, we show that re-allocation of proline from cytosol to vacuole was similar in the pyrroline-5-carboxylate synthase 2–1 (p5cs2–1) mutant and the pgm mutant, and caused similar reduction of basal freezing tolerance. In contrast, the starch excess 1–1 mutant (sex1-1) had even lower freezing tolerance than pgm, but did not affect sub-cellular localization of proline. Freezing sensitivity of sex1-1 mutants affected primarily the photosynthetic electron transport and was enhanced in a sex1-1::p5cs2–1 double mutant. These findings indicate that several independent factors determine basal freezing tolerance. In a pgm::p5cs2–1 double mutant, freezing sensitivity and proline allocation to the vacuole were the same as in the parental lines, indicating that the lack of cytosolic proline was the common cause of reduced basal freezing tolerance in both mutants. We conclude that cytosolic proline is an important factor in freezing tolerance of non-acclimated plants. This article is protected by copyright. All rights reserved.}, author = {Hoermiller, Imke I. and Funck, Dietmar and Schönewolf, Lilli and May, Henrik and Heyer, Arnd G.}, doi = {https://doi.org/10.1111/pce.14196}, eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14196}, journal = {Plant, Cell \& Environment}, title = {Cytosolic proline is required for basal freezing tolerance in Arabidopsis}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14196}, year = 2022 }
Link
https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14196
Küstner, L., Fürtauer, L., Weckwerth, W., Nägele, T., Heyer, A.G.: Subcellular dynamics of proteins and metabolites under abiotic stress reveal deferred response of the Arabidopsis thaliana hexokinase‐1 mutant gin2‐1 to high light. Plant Journal. 100, 456–472 (2019). https://doi.org/10.1111/tpj.14491.
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@article{kustner2019subcellular, abstract = {Stress responses in plants imply spatio‐temporal changes in enzymes and metabolites, including subcellular compartment‐specific reallocation processes triggered by sudden changes in environmental parameters. To investigate interactions of primary metabolism with abiotic stress, the gin2‐1 mutant, defective in the sugar sensor hexokinase 1 (HXK1) was compared to its wildtype Landsberg erecta (Ler) based on time resolved, compartment specific metabolome and proteome data obtained over a full diurnal cycle. The high light sensitive gin2‐1 mutant was substantially delayed in subcellular redistribution of metabolites upon stress, and this correlated with a massive reduction in proteins belonging to the ATP producing electron transport chain under high light, while fewer changes occurred in the cold. In the wildtype, compounds specifically protecting individual compartments could be identified, e.g. maltose and raffinose in plastids, myo‐inositol in mitochondria, but gin2‐1 failed to recruit these substances to the respective compartments, or responded only slowly to high irradiance. No such delay was obtained in the cold. At the whole‐cell level, concentrations of the amino acids, glycine and serine, provided strong evidence for an important role of the photorespiratory pathway during stress exposure, and different subcellular allocation of serine may contribute to the slow growth of the gin2‐1 mutant under high irradiance.}, author = {Küstner, Lisa and Fürtauer, Lisa and Weckwerth, Wolfram and Nägele, Thomas and Heyer, Arnd G.}, doi = {10.1111/tpj.14491}, journal = {Plant Journal}, month = {08}, pages = {456-472}, title = {Subcellular dynamics of proteins and metabolites under abiotic stress reveal deferred response of the Arabidopsis thaliana hexokinase‐1 mutant gin2‐1 to high light}, url = {https://doi.org/10.1111/tpj.14491}, volume = 100, year = 2019 }
Link
https://doi.org/10.1111/tpj.14491
Mishra, K.B., Mishra, A., Kubásek, J., Urban, O., Heyer, A.G., Govindjee: Low temperature induced modulation of photosynthetic induction in non-acclimated and cold-acclimated Arabidopsis thaliana: chlorophyll a fluorescence and gas-exchange measurements. Photosynthesis Research. (2018). https://doi.org/10.1007/s11120-018-0588-7.
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@article{Mishra2018, abstract = {Cold acclimation modifies the photosynthetic machinery and enables plants to survive at sub-zero temperatures, whereas in warm habitats, many species suffer even at non-freezing temperatures. We have measured chlorophyll a fluorescence (ChlF) and CO2 assimilation to investigate the effects of cold acclimation, and of low temperatures, on a cold-sensitive Arabidopsis thaliana accession C24. Upon excitation with low intensity (40 {\textmu}mol photons m−{\thinspace}2 s−{\thinspace}1){\thinspace}{\textasciitilde}{\thinspace}620 nm light, slow (minute range) ChlF transients, at {\textasciitilde}{\thinspace}22 {\textdegree}C, showed two waves in the SMT phase (S, semi steady-state; M, maximum; T, terminal steady-state), whereas CO2 assimilation showed a linear increase with time. Low-temperature treatment (down to −{\thinspace}1.5 {\textdegree}C) strongly modulated the SMT phase and stimulated a peak in the CO2 assimilation induction curve. We show that the SMT phase, at {\textasciitilde}{\thinspace}22 {\textdegree}C, was abolished when measured under high actinic irradiance, or when 3-(3, 4-dichlorophenyl)-1, 1- dimethylurea (DCMU, an inhibitor of electron flow) or methyl viologen (MV, a Photosystem I (PSI) electron acceptor) was added to the system. Our data suggest that stimulation of the SMT wave, at low temperatures, has multiple reasons, which may include changes in both photochemical and biochemical reactions leading to modulations in non-photochemical quenching (NPQ) of the excited state of Chl, ``state transitions,'' as well as changes in the rate of cyclic electron flow through PSI. Further, we suggest that cold acclimation, in accession C24, promotes ``state transition'' and protects photosystems by preventing high excitation pressure during low-temperature exposure.}, author = {Mishra, Kumud B. and Mishra, Anamika and Kub{\'a}sek, Ji{\v{r}}{\'i} and Urban, Otmar and Heyer, Arnd G. and Govindjee}, day = 10, doi = {10.1007/s11120-018-0588-7}, issn = {1573-5079}, journal = {Photosynthesis Research}, month = {10}, title = {Low temperature induced modulation of photosynthetic induction in non-acclimated and cold-acclimated Arabidopsis thaliana: chlorophyll a fluorescence and gas-exchange measurements}, url = {https://doi.org/10.1007/s11120-018-0588-7}, year = 2018 }
Link
https://doi.org/10.1007/s11120-018-0588-7
Hoermiller, I.I., Ruschhaupt, M., Heyer, A.G.: Mechanisms of frost resistance in Arabidopsis thaliana. Planta. 248, 827–835 (2018). https://doi.org/10.1007/s00425-018-2939-1.
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@article{Hoermiller2018, abstract = {Freezing resistance strategies vary in Arabidopsis depending on origin. Southern accessions may avoid or tolerate freezing, while northern ones are always tolerant and reduce the proportion of freezable tissue water during acclimation.}, author = {Hoermiller, Imke I. and Ruschhaupt, Moritz and Heyer, Arnd G.}, day = 01, doi = {10.1007/s00425-018-2939-1}, issn = {1432-2048}, journal = {Planta}, month = {10}, number = 4, pages = {827-835}, title = {Mechanisms of frost resistance in Arabidopsis thaliana}, url = {https://doi.org/10.1007/s00425-018-2939-1}, volume = 248, year = 2018 }
Link
https://doi.org/10.1007/s00425-018-2939-1
Hoermiller, I.I., Naegele, T., Augustin, H., Stutz, S., Weckwerth, W., Heyer, A.G.: Subcellular reprogramming of metabolism during cold acclimation in Arabidopsis thaliana. Plant Cell Environ. 40, 602–610 (2017). https://doi.org/10.1111/pce.12836.
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@article{Hoermiller2017, abstract = {Abstract Metabolite changes in plant leaves during exposure to low temperatures involve re‐allocation of a large number of metabolites between sub‐cellular compartments. Therefore, metabolite determination at the whole cell level may be insufficient for interpretation of the functional significance of cellular compounds. To investigate the cold‐induced metabolite dynamics at the level of individual sub‐cellular compartments, an integrative platform was developed that combines quantitative metabolite profiling by gas chromatography coupled to mass spectrometry (GC‐MS) with the non‐aqueous fractionation technique allowing separation of cytosol, vacuole and the plastidial compartment. Two mutants of Arabidopsis thaliana representing antipodes in the diversion of carbohydrate metabolism between sucrose and starch were compared to Col‐0 wildtype before and after cold acclimation to investigate interactions of cold acclimation with subcellular re‐programming of metabolism. A multivariate analysis of the data set revealed dominant effects of compartmentation on metabolite concentrations that were modulated by environmental condition and genetic determinants. While for both, the starchless mutant of plastidial phospho‐gluco mutase (pgm) and a mutant defective in sucrose‐phosphate synthase A1, metabolic constraints, especially at low temperature, could be uncovered based on subcellularly resolved metabolite profiles, only pgm had lowered freezing tolerance. Metabolic profiles of pgm point to redox imbalance as a possible reason for reduced cold acclimation capacity.}, author = {Hoermiller, Imke I. and Naegele, Thomas and Augustin, Hanna and Stutz, Simon and Weckwerth, Wolfram and Heyer, Arnd G.}, doi = {10.1111/pce.12836}, eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.12836}, journal = {Plant Cell Environ.}, number = 5, pages = {602-610}, title = {Subcellular reprogramming of metabolism during cold acclimation in Arabidopsis thaliana}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12836}, volume = 40, year = 2017 }
Link
https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12836
Xu, E., Vaathera, L., Hörak, H., Hincha, D.K., Heyer, A.G., Brosche, M.: Quantitative trait loci mapping and transcriptome analysis reveal candidate genes regulating the response to ozone in Arabidopsis thaliana. Plant, Cell & Environment. 38, 1418–1433 (2015). https://doi.org/10.1111/pce.12499.
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@article{10.1111/pce.12499, abstract = {Abstract As multifaceted molecules, reactive oxygen species (ROS) are known to accumulate in response to various stresses. Ozone (O3) is an air pollutant with detrimental effect on plants and O3 can also be used as a tool to study the role of ROS in signalling. Genetic variation of O3 sensitivity in different Arabidopsis accessions highlights the complex genetic architecture of plant responses to ROS. To investigate the genetic basis of O3 sensitivity, a recombinant inbred line (RIL) population between two Arabidopsis accessions with distinct O3 sensitivity, C24 (O3 tolerant) and Te (O3 sensitive) was used for quantitative trait loci (QTL) mapping. Through analysis of QTL mapping combined with transcriptome changes in response to O3, we identified three causal QTLs and several potential candidate genes regulating the response to O3. Based on gene expression data, water loss and stomatal conductance measurement, we found that a combination of relatively low stomatal conductance and constitutive activation of salicylic acid (SA)-mediated defence signalling were responsible for the O3 tolerance in C24. Application of exogenous SA prior to O3 exposure can mimic the constitutive SA signalling in C24 and could attenuate O3-induced leaf damage in the sensitive Arabidopsis accessions Te and Cvi-0.}, author = {Xu, Enjun and Vaathera, Lauri and Hörak, Hanna and Hincha, Dirk K. and Heyer, Arnd G. and Brosche, Mikael}, doi = {10.1111/pce.12499}, eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.12499}, journal = {Plant, Cell \& Environment}, number = 7, pages = {1418-1433}, title = {Quantitative trait loci mapping and transcriptome analysis reveal candidate genes regulating the response to ozone in Arabidopsis thaliana}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12499}, volume = 38, year = 2015 }
Link
https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12499
Mishra, A., Heyer, A., Mishra, K.: Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated Arabidopsis thaliana accessions. Plant Methods. 10, 38 (2014). https://doi.org/10.1186/1746-4811-10-38.
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@article{Mishra2014, abstract = {BACKGROUND:An easy and non-invasive method for measuring plant cold tolerance is highly valuable to instigate research targeting breeding of cold tolerant crops. Traditional methods are labor intensive, time-consuming and thereby of limited value for large scale screening. Here, we have tested the capacity of chlorophyll a fluorescence (ChlF) imaging based methods for the first time on intact whole plants and employed advanced statistical classifiers and feature selection rules for finding combinations of images able to discriminate cold tolerant and cold sensitive plants.RESULTS:ChlF emission from intact whole plant rosettes of nine Arabidopsis thaliana accessions was measured for (1) non-acclimated (NAC, six week old plants grown at room temperature), (2) cold acclimated (AC, NAC plants acclimated at 4degreesC for two weeks), and (3) sub-zero temperature (ST) treated (STT, AC plants treated at -4degreesC for 8h in dark) states. Cold acclimation broadened the slow phase of ChlF transients in cold sensitive (Co, C24, Can and Cvi) A. thaliana accessions. Similar broadening in the slow phase of ChlF transients was observed in cold tolerant (Col, Rsch, and Te) plants following ST treatments. ChlF parameters: maximum quantum yield of PSII photochemistry (FV/FM) and fluorescence decrease ratio (RFD) well categorized the cold sensitive and tolerant plants when measured in STT state. We trained a range of statistical classifiers with the sequence of captured ChlF images and selected a high performing quadratic discriminant classifier (QDC) in combination with sequential forward floating selection (SFFS) feature selection methods and found that linear combination of three images showed a reasonable contrast between cold sensitive and tolerant A. thaliana accessions for AC as well as for STT states.CONCLUSIONS:ChlF transients measured for an intact whole plant is important for understanding the impact of cold acclimation on photosynthetic processes. Combinatorial imaging combined with statistical classifiers and feature selection methods worked well for the screening of cold tolerance without exposing plants to sub-zero temperatures. This opens up new possibilities for high-throughput monitoring of whole plants cold tolerance via easy and fully non-invasive means.}, author = {Mishra, Anamika and Heyer, Arnd and Mishra, Kumud}, doi = {10.1186/1746-4811-10-38}, issn = {1746-4811}, journal = {Plant Methods}, number = 1, pages = 38, pubmedid = {25400689}, title = {Chlorophyll fluorescence emission can screen cold tolerance of cold acclimated \textit{Arabidopsis thaliana} accessions}, url = {http://www.plantmethods.com/content/10/1/38}, volume = 10, year = 2014 }
Link
http://www.plantmethods.com/content/10/1/38
Meissner, M., Orsini, E., Ruschhaupt, M., Melchinger, A.E., Hincha, D.K., Heyer, A.G.: Mapping quantitative trait loci for freezing tolerance in a recombinant inbred line population of Arabidopsis thaliana accessions Tenela and C24 reveals REVEILLE1 as negative regulator of cold acclimation. Plant Cell Environ. 36, 1256–1267 (2013). https://doi.org/10.1111/pce.12054.
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@article{Meissner2013, abstract = {The ability to increase freezing tolerance when exposed to low temperatures is a property of many plant species from temperate climates and involves a wide array of metabolic adjustments and changes in gene expression. In Arabidopsis thaliana, natural accessions show high variation in their acclimation capacity, and freezing tolerance correlates with natural habitat temperatures. To investigate the genetic basis of this variation, a recombinant inbred line population from reciprocal crosses between the accessions C24 and Tenela (Te), showing large variation in tolerance, was established. Over 250 recombinant inbred lines were genotyped for 69 single nucleotide polymorphism markers in a linkage map with 391.9 centimorgans (cM) and phenotyped for their freezing tolerance using the electrolyte leakage method that reports cell damage after a freeze-thaw cycle. Mapping of quantitative trait loci (QTL) for acclimated plants revealed three QTL regions on chromosomes 2, 4 and 5. Based on gene expression data, QTL regions were screened for genes differentially responding to low temperature in C24 and Te. Among the candidate genes, the Myb family transcription factor REVEILLE1 (At5g17300) on chromosome 5 was identified as a novel negative regulator of freezing tolerance in Arabidopsis}, author = {Meissner, M. and Orsini, E. and Ruschhaupt, M. and Melchinger, A.E. and Hincha, Dirk K. and Heyer, Arnd G.}, doi = {10.1111/pce.12054}, issn = {1365-3040}, journal = {Plant Cell Environ.}, month = {02}, owner = {heyer}, pages = {1256-1267}, refid = {48}, title = {Mapping quantitative trait loci for freezing tolerance in a recombinant inbred line population of \textit{Arabidopsis thaliana} accessions Tenela and C24 reveals REVEILLE1 as negative regulator of cold acclimation}, url = {http://dx.doi.org/10.1111/pce.12054}, volume = 36, year = 2013 }
Link
http://dx.doi.org/10.1111/pce.12054
Distelbarth, H., Nägele, T., Heyer, A.: Responses of antioxidant enzymes to cold and highlight are not correlated to freezing tolerance in natural accessions of Arabidopsis thaliana. Plant Biol. 15, 982--990 (2013). https://doi.org/10.1111/j.1438-8677.2012.00718.x.
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@article{Distelbarth2013, abstract = {Low temperatures and high light cause imbalances in primary and secondary reactions of photosynthesis and thus, can result in oxidative stress. Plants employ a range of low molecular weight antioxidants and antioxidant enzymes to prevent oxidative damage, and antioxidant defense is considered an important component of stress tolerance. To figure out whether oxidative stress and antioxidant defense are a key factors defining the differential cold acclimation capacities of natural accessions of the model plant �Arabidopsis thaliana�, we investigated hydrogen peroxide production, antioxidant enzyme activity and lipid peroxidation during a time course of cold treatment and exposure to high light in four differentially cold tolerant natural accessions of �Arabidopsis� (C24, Nd, Rsch, Te) that span the European distribution range of the species. All accessions except Rsch from Russia had elevated hydrogen peroxide in the cold, indicating that production of reactive oxygen species is part of the cold response in �Arabidopsis�. Glutathione reductase activity increased in all but Rsch, while ascorbate peroxidase and superoxide dismutase were unchanged and catalase decreased in all but Rsch. Under high light, the Scandinavian accession Te had elevated levels of hydrogen peroxide. Te appeared most sensitive to oxidative stress, having higher malondialdehyde (MDA) levels in the cold and under high light, while only high light caused elevated MDA in the other accessions. Although most freezing tolerant, Te displayed the highest sensitivity towards oxidative stress. No correlation was found between freezing tolerance and activity of antioxidant enzymes in the four accessions investigated, arguing against a key role of antioxidant defense in the differential cold acclimation capacities of the �Arabidopsis� accessions.}, author = {Distelbarth, H. and Nägele, T. and Heyer, A.}, doi = {10.1111/j.1438-8677.2012.00718.x}, journal = {Plant Biol.}, owner = {heyer}, pages = {982--990}, refid = {49}, title = {Responses of antioxidant enzymes to cold and highlight are not correlated to freezing tolerance in natural accessions of Arabidopsis thaliana}, volume = 15, year = 2013 }
Nägele, T., Heyer, A.G.: Approximating subcellular organisation of carbohydrate metabolism during cold acclimation in different natural accessions of Arabidopsis thaliana. New Phytol. 198, 777--787 (2013). https://doi.org/10.1111/nph.12201.
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@article{Naegele2013, abstract = {Accessions of Arabidopsis thaliana originating from climatically different habitats show different levels of cold acclimation when exposed to low temperatures. The central carbohydrate metabolism plays a crucial role during this acclimation. Subcellular distribution of carbohydrates over the compartments cytosol, vacuole and plastids, and putative interactions of the compartments, are analyzed in three differentially cold-tolerant accessions of Arabidopsis thaliana, originating from the Iberian Peninsula (C24), Russia (Rschew) and Scandinavia (Tenela), respectively. * Subcellular carbohydrate concentrations were determined by applying the nonaqueous fractionation technique. Mathematical modeling and steady-state simulation was used to analyse the metabolic homeostasis during cold exposure. * In all accessions, the initial response to cold exposure was a significant increase of plastidial and cytosolic sucrose concentrations. Raffinose accumulated in all cellular compartments of cold-tolerant accessions with a delay of 3-�d, indicating that raffinose accumulation is a long-term component of cold acclimation. Minimal rates of metabolite transport permitting steady-state simulations of metabolite concentrations correlated with cold tolerance, indicating an important role of subcellular re-distribution of metabolites during cold acclimation. * A highly regulated interplay of enzymatic reactions and intracellular transport processes appears to be a prerequisite for maintaining carbohydrate homeostasis during cold exposure and allowing cold acclimation in Arabidopsis thaliana}, author = {Nägele, Thomas and Heyer, Arnd G.}, doi = {10.1111/nph.12201}, issn = {1469-8137}, journal = {New Phytol.}, month = {03}, owner = {heyer}, pages = {777--787}, refid = {45}, title = {Approximating subcellular organisation of carbohydrate metabolism during cold acclimation in different natural accessions of \textit{Arabidopsis thaliana}}, url = {http://dx.doi.org/10.1111/nph.12201}, volume = 198, year = 2013 }
Link
http://dx.doi.org/10.1111/nph.12201
Nägele, T., Stutz, S., Hörmiller, I.I., Heyer, A.G.: Identification of a metabolic bottleneck for cold acclimation in Arabidopsis thaliana. Plant J. 72, 102–114 (2012). https://doi.org/10.1111/j.1365-313X.2012.05064.x.
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@article{Naegele2012a, abstract = {Central carbohydrate metabolism of Arabidopsis thaliana is known to play a crucial role during cold acclimation and the acquisition of freezing tolerance. During cold exposure, many carbohydrates accumulate and a new metabolic homeostasis evolves. In the present study, we analyse the diurnal dynamics of carbohydrate homeostasis before and after cold exposure in three natural accessions showing distinct cold acclimation capacity. Diurnal dynamics of soluble carbohydrates were found to be significantly different in cold-sensitive and cold-tolerant accessions. Although experimentally determined maximum turnover rates for sucrose phosphate synthase in cold-acclimated leaves were higher for cold-tolerant accessions, model simulations of diurnal carbohydrate dynamics revealed similar fluxes. This implied a significantly higher capacity for sucrose synthesis in cold-tolerant than cold-sensitive accessions. Based on this implication resulting from mathematical model simulation, a critical temperature for sucrose synthesis was calculated using the Arrhenius equation and experimentally validated in the cold-sensitive accession C24. At the critical temperature suggested by model simulation, an imbalance in photosynthetic carbon fixation ultimately resulting in oxidative stress was observed. It is therefore concluded that metabolic capacities at least in part determine the ability of accessions of Arabidopsis thaliana to cope with changes in environmental conditions}, author = {Nägele, T. and Stutz, Simon and Hörmiller, Imke I. and Heyer, Arnd G.}, doi = {10.1111/j.1365-313X.2012.05064.x}, issn = {1365-313X}, journal = {Plant J.}, month = {05}, number = 102, owner = {heyer}, pages = {102-114}, refid = {44}, title = {Identification of a metabolic bottleneck for cold acclimation in \textit{Arabidopsis thaliana}}, url = {http://dx.doi.org/10.1111/j.1365-313X.2012.05064.x}, volume = 72, year = 2012 }
Link
http://dx.doi.org/10.1111/j.1365-313X.2012.05064.x
Iftime, D., Hannah, M.A., Peterbauer, T., Heyer, A.G.: Stachyose in the cytosol does not influence freezing tolerance of transgenic Arabidopsis expressing stachyose synthase from adzuki bean. Plant Science. 180, 24--30 (2011). https://doi.org/10.1016/j.plantsci.2010.07.012.
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@article{Iftime_2011, author = {Iftime, Dumitrita and Hannah, Matthew A. and Peterbauer, Thomas and Heyer, Arnd G.}, doi = {10.1016/j.plantsci.2010.07.012}, journal = {Plant Science}, month = {01}, number = 1, pages = {24--30}, publisher = {Elsevier {BV}}, title = {Stachyose in the cytosol does not influence freezing tolerance of transgenic Arabidopsis expressing stachyose synthase from adzuki bean}, url = {https://doi.org/10.1016%2Fj.plantsci.2010.07.012}, volume = 180, year = 2011 }
Link
https://doi.org/10.1016%2Fj.plantsci.2010.07.012
Mishra, A., Mishra, K.B., Hoermiller, I.I., Heyer, A.G., Nedbal, L.: Chlorophyll fluorescence emission as a reporter on cold tolerance in Arabidopsis thaliana accessions. Plant Signaling Behav. 6, 301--310 (2011). https://doi.org/10.4161/psb.6.2.15278.
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@article{Mishra2011, abstract = {Non-invasive, high-throughput screening methods are valuable tools in breeding for abiotic stress tolerance in plants. Optical signals such as chlorophyll fluorescence emission can be instrumental in developing new screening techniques. In order to examine the potential of chlorophyll fluorescence to reveal plant tolerance to low temperatures, we used a collection of nine Arabidopsis thaliana accessions and compared their fluorescence features with cold tolerance quantified by the well established electrolyte leakage method on detached leaves. We found that, during progressive cooling, the minimal chlorophyll fluorescence emission rose strongly and that this rise was highly dependent on the cold tolerance of the accessions. Maximum quantum yield of PSII photochemistry and steady state fluorescence normalized to minimal fluorescence were also highly correlated to the cold tolerance measured by the electrolyte leakage method. In order to further increase the capacity of the fluorescence detection to reveal the low temperature tolerance, we applied combinatorial imaging that employs plant classification based on multiple fluorescence features. We found that this method, by including the resolving power of several fluorescence features, can be well employed to detect cold tolerance already at mild sub-zero temperatures. Therefore, there is no need to freeze the screened plants to the largely damaging temperatures of around -15�C. This, together with the method's easy applicability, represents a major advantage of the fluorescence technique over the conventional electrolyte leakage method.}, author = {Mishra, A. and Mishra, K.B. and Hoermiller, I.I. and Heyer, A.G. and Nedbal, L.}, doi = {10.4161/psb.6.2.15278}, journal = {Plant Signaling Behav.}, month = {02}, number = 2, owner = {heyer}, pages = {301--310}, refid = {40}, title = {Chlorophyll fluorescence emission as a reporter on cold tolerance in \textit{Arabidopsis thaliana} accessions}, url = {https://www.tandfonline.com/doi/abs/10.4161/psb.6.2.15278}, volume = 6, year = 2011 }
Link
https://www.tandfonline.com/doi/abs/10.4161/psb.6.2.15278
Knaupp, M., Mishra, K.B., Nedbal, L., Heyer, A.G.: Evidence for a role of raffinose in stabilizing photosystem II during freeze--thaw cycles. Planta. 234, 477--486 (2011). https://doi.org/10.1007/s00425-011-1413-0.
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@article{Knaupp2011, abstract = {A role of non-reducing sugars like sucrose and raffinose in the protection of plant cells against damage during freezing has been proposed for many species, but reports on physiological effects are conflicting. Non-aqueous fractionation of mesophyll cell compartments in Arabidopsis thaliana was used to show that sucrose and raffinose accumulate in plastids during low temperatures, pointing to a physiological role in protecting the photosynthetic apparatus. Comparing a previously described raffinose synthase (RS) mutant of A. thaliana with its corresponding wild type, accession Col-0, revealed that a lack of raffinose has no effect on electrolyte leakage from leaf cells after freeze--thaw cycles, supporting that raffinose is not essential for protecting the plasma membrane. However, in situ chlorophyll fluorescence showed that maximum quantum yield of PS II photochemistry (F v/F m) and other fluorescence parameters of cold acclimated leaves subjected to freeze--thaw cycles were significantly lower in the raffinose synthase mutant than in the corresponding wild type, indicating that raffinose is involved in stabilizing PS II of cold acclimated leaf cells against damage during freezing.}, author = {Knaupp, Markus and Mishra, Kumud B. and Nedbal, Ladislav and Heyer, Arnd G.}, day = 01, doi = {10.1007/s00425-011-1413-0}, issn = {1432-2048}, journal = {Planta}, month = {09}, number = 3, pages = {477--486}, title = {Evidence for a role of raffinose in stabilizing photosystem II during freeze--thaw cycles}, url = {https://doi.org/10.1007/s00425-011-1413-0}, volume = 234, year = 2011 }
Link
https://doi.org/10.1007/s00425-011-1413-0
Nägele, T., Kandel, B.A., Frana, S., Meissner, M., Heyer, A.G.: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana. FEBS J. 278, 506--518 (2011). https://doi.org/10.1111/j.1742-4658.2010.07971.x.
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@article{Naegele2011, abstract = {Low temperature is an important environmental factor affecting the performance and distribution of plants. During the so-called process of cold acclimation, many plants are able to develop low-temperature tolerance, associated with the reprogramming of a large part of their metabolism. In this study, we present a systems biology approach based on mathematical modelling to determine interactions between the reprogramming of central carbohydrate metabolism and the development of freezing tolerance in two accessions of Arabidopsis thaliana. Different regulation strategies were observed for (a) photosynthesis, (b) soluble carbohydrate metabolism and (c) enzyme activities of central metabolite interconversions. Metabolism of the storage compound starch was found to be independent of accession-specific reprogramming of soluble sugar metabolism in the cold. Mathematical modelling and simulation of cold-induced metabolic reprogramming indicated major differences in the rates of interconversion between the pools of hexoses and sucrose, as well as the rate of assimilate export to sink organs. A comprehensive overview of interconversion rates is presented, from which accession-specific regulation strategies during exposure to low temperature can be derived. We propose this concept as a tool for predicting metabolic engineering strategies to optimize plant freezing tolerance. We confirm that a significant improvement in freezing tolerance in plants involves multiple regulatory instances in sucrose metabolism, and provide evidence for a pivotal role of sucrose-hexose interconversion in increasing the cold acclimation output}, author = {Nägele, T. and Kandel, B.A. and Frana, S. and Meissner, M. and Heyer, A.G.}, comment = {J}, doi = {10.1111/j.1742-4658.2010.07971.x}, issn = {1742-464X}, journal = {FEBS J.}, number = 3, owner = {heyer}, pages = {506--518}, privnote = {J}, refid = {36}, title = {A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana}, url = {/brokenurl#ISI:000286328400009}, volume = 278, year = 2011 }
Link
/brokenurl#ISI:000286328400009
Livingston, D.P., Hincha, D.K., Heyer, A.G.: Fructan and its relationship to abiotic stress tolerance in plants. Cellular and Molecular Life Sciences. 66, 2007--2023 (2009). https://doi.org/10.1007/s00018-009-0002-x.
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@article{Livingston2009, abstract = {Numerous studies have been published that attempted to correlate fructan concentrations with freezing and drought tolerance. Studies investigating the effect of fructan on liposomes indicated that a direct interaction between membranes and fructan was possible. This new area of research began to move fructan and its association with stress beyond mere correlation by confirming that fructan has the capacity to stabilize membranes during drying by inserting at least part of the polysaccharide into the lipid headgroup region of the membrane. This helps prevent leakage when water is removed from the system either during freezing or drought. When plants were transformed with the ability to synthesize fructan, a concomitant increase in drought and/or freezing tolerance was confirmed. These experiments indicate that besides an indirect effect of supplying tissues with hexose sugars, fructan has a direct protective effect that can be demonstrated by both model systems and genetic transformation.}, author = {Livingston, David P. and Hincha, Dirk K. and Heyer, Arnd G.}, day = 01, doi = {10.1007/s00018-009-0002-x}, issn = {1420-9071}, journal = {Cellular and Molecular Life Sciences}, month = {07}, number = 13, pages = {2007--2023}, title = {Fructan and its relationship to abiotic stress tolerance in plants}, url = {https://doi.org/10.1007/s00018-009-0002-x}, volume = 66, year = 2009 }
Link
https://doi.org/10.1007/s00018-009-0002-x
Korn, M., Peterek, S., Mock, H.P., Heyer, A.G., Hincha, D.K.: Heterosis in the freezing tolerance, and sugar and flavonoid contents of crosses between Arabidopsis thaliana accessions of widely varying freezing tolerance. Plant Cell Environ. 31, 813--827 (2008). https://doi.org/10.1111/j.1365-3040.2008.01800.x.
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@article{Korn2008, abstract = {Heterosis is defined as the increased vigour of hybrids in comparison to their parents. We investigated 24 F-1 hybrid lines of Arabidopsis thaliana generated by reciprocally crossing either C24 or Col with six other parental accessions (Can, Co, Cvi, Ler, Rsch, Te) that differ widely in their freezing tolerance. The crosses differed in the degree of heterosis for freezing tolerance, both in the non-acclimated state and after a 14 d cold acclimation period. Crosses with C24 showed more heterosis than crosses with Col, and heterosis was stronger in acclimated than in non-acclimated plants. Leaf content of soluble sugars and proline showed more deviation from mid-parent values in crosses involving C24 than in those involving Col, and deviations were larger in acclimated than in non-acclimated plants. There were significant correlations between the content of different sugars and leaf freezing tolerance, as well as between heterosis effects in freezing tolerance and sugar content. Flavonoid content and composition varied between accessions, and between non-acclimated and acclimated plants. In the crosses, large deviations from the mid-parent values in the contents of different flavonols occurred, and there were strikingly strong correlations between both flavonol content and freezing tolerance, and between heterosis effects in freezing tolerance and flavonol content. [References: 48]}, author = {Korn, M. and Peterek, S. and Mock, H.P. and Heyer, A.G. and Hincha, D.K.}, comment = {DB - Current Contents PT - Article <2>}, doi = {10.1111/j.1365-3040.2008.01800.x}, journal = {Plant Cell Environ.}, month = {06}, number = 6, owner = {heyer}, pages = {813--827}, privnote = {DB - Current Contents PT - Article <2>}, refid = {1}, title = {Heterosis in the freezing tolerance, and sugar and flavonoid contents of crosses between \textit{Arabidopsis thaliana} accessions of widely varying freezing tolerance}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-3040.2008.01800.x}, volume = 31, year = 2008 }
Link
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-3040.2008.01800.x
Korn, M., Hincha, D., Heyer, A.: Heterosis in the frost tolerance of crosses between different Arabidopsis thaliana accessions. 146, S153 (2007). https://doi.org/10.1016/j.cbpa.2007.01.312.
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@article{2007, author = {Korn, M. and Hincha, D. and Heyer, A.}, doi = {10.1016/j.cbpa.2007.01.312}, month = {04}, number = 4, pages = {S153}, publisher = {Elsevier {BV}}, title = {Heterosis in the frost tolerance of crosses between different Arabidopsis thaliana accessions}, url = {https://doi.org/10.1016%2Fj.cbpa.2007.01.312}, volume = 146, year = 2007 }
Link
https://doi.org/10.1016%2Fj.cbpa.2007.01.312
Hincha, D.K., Livingston, D.P., Premakumar, R., Zuther, E., Obel, N., Cacela, C., Heyer, A.G.: Fructans from oat and rye: Composition and effects on membrane stability during drying. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1768, 1611--1619 (2007). https://doi.org/10.1016/j.bbamem.2007.03.011.
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@article{Hincha_2007, author = {Hincha, Dirk K. and Livingston, David P. and Premakumar, Ramaswamy and Zuther, Ellen and Obel, Nicolai and Cacela, Constan{\c{c}}a and Heyer, Arnd G.}, doi = {10.1016/j.bbamem.2007.03.011}, journal = {Biochimica et Biophysica Acta ({BBA}) - Biomembranes}, month = {06}, number = 6, pages = {1611--1619}, publisher = {Elsevier {BV}}, title = {Fructans from oat and rye: Composition and effects on membrane stability during drying}, url = {https://doi.org/10.1016%2Fj.bbamem.2007.03.011}, volume = 1768, year = 2007 }
Link
https://doi.org/10.1016%2Fj.bbamem.2007.03.011
Hincha, D., Hannah, M., Le, M., Heyer, A.: Plant freezing tolerance: From gene expression to functional adaptation. 146, S151 (2007). https://doi.org/10.1016/j.cbpa.2007.01.306.
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@article{2007, author = {Hincha, D. and Hannah, M. and Le, M. and Heyer, A.}, doi = {10.1016/j.cbpa.2007.01.306}, month = {04}, number = 4, pages = {S151}, publisher = {Elsevier {BV}}, title = {Plant freezing tolerance: From gene expression to functional adaptation}, url = {https://doi.org/10.1016%2Fj.cbpa.2007.01.306}, volume = 146, year = 2007 }
Link
https://doi.org/10.1016%2Fj.cbpa.2007.01.306
Hannah, M.A., Wiese, D., Freund, S., Fiehn, O., Heyer, A.G., Hincha, D.K.: Natural genetic variation of freezing tolerance in Arabidopsis. Plant Physiol. 142, 98--112 (2006). https://doi.org/10.1104/pp.106.081141.
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@article{Hannah2006, abstract = {Low temperature is a primary determinant of plant growth and survival. Using accessions of Arabidopsis (Arabidopsis thaliana) originating from Scandinavia to the Cape Verde Islands, we show that freezing tolerance of natural accessions correlates with habitat winter temperatures, identifying low temperature as an important selective pressure for Arabidopsis. Combined metabolite and transcript profiling show that during cold exposure, global changes of transcripts, but not of metabolites, correlate with the ability of Arabidopsis to cold acclimate. There are, however, metabolites and transcripts, including several transcription factors, that correlate with freezing tolerance, indicating regulatory pathways that may be of primary importance for this trait. These data identify that enhanced freezing tolerance is associated with the down-regulation of photosynthesis and hormonal responses and the induction of flavonoid metabolism, provide evidence for naturally increased nonacclimated freezing tolerance due to the constitutive activation of the C-repeat binding factors pathway, and identify candidate transcriptional regulators that correlate with freezing tolerance. [References: 61]}, author = {Hannah, M.A. and Wiese, D. and Freund, S. and Fiehn, O. and Heyer, A.G. and Hincha, D.K.}, comment = {DB - Current Contents PT - Article <6>}, doi = {10.1104/pp.106.081141}, journal = {Plant Physiol.}, month = {09}, number = 1, owner = {heyer}, pages = {98--112}, privnote = {DB - Current Contents PT - Article <6>}, refid = {5}, title = {Natural genetic variation of freezing tolerance in \textit{Arabidopsis}}, url = {http://www.plantphysiol.org/content/142/1/98}, volume = 142, year = 2006 }
Link
http://www.plantphysiol.org/content/142/1/98
Hannah, M.A., Heyer, A.G., Hincha, D.K.: A Global Survey of Gene Regulation during Cold Acclimation in Arabidopsis thaliana. PLOS Genetics. 1, (2005). https://doi.org/10.1371/journal.pgen.0010026.
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@article{10.1371/journal.pgen.0010026, abstract = {Many temperate plant species such as Arabidopsis thaliana are able to increase their freezing tolerance when exposed to low, nonfreezing temperatures in a process called cold acclimation. This process is accompanied by complex changes in gene expression. Previous studies have investigated these changes but have mainly focused on individual or small groups of genes. We present a comprehensive statistical analysis of the genome-wide changes of gene expression in response to 14 d of cold acclimation in Arabidopsis, and provide a large-scale validation of these data by comparing datasets obtained for the Affymetrix ATH1 Genechip and MWG 50-mer oligonucleotide whole-genome microarrays. We combine these datasets with existing published and publicly available data investigating Arabidopsis gene expression in response to low temperature. All data are integrated into a database detailing the cold responsiveness of 22,043 genes as a function of time of exposure at low temperature. We concentrate our functional analysis on global changes marking relevant pathways or functional groups of genes. These analyses provide a statistical basis for many previously reported changes, identify so far unreported changes, and show which processes predominate during different times of cold acclimation. This approach offers the fullest characterization of global changes in gene expression in response to low temperature available to date.}, author = {Hannah, Matthew A and Heyer, Arnd G and Hincha, Dirk K}, doi = {10.1371/journal.pgen.0010026}, journal = {PLOS Genetics}, month = {08}, number = 2, publisher = {Public Library of Science}, title = {A Global Survey of Gene Regulation during Cold Acclimation in Arabidopsis thaliana}, url = {https://doi.org/10.1371/journal.pgen.0010026}, volume = 1, year = 2005 }
Link
https://doi.org/10.1371/journal.pgen.0010026
Zuther, E., Büchel, K., Hundertmark, M., Stitt, M., Hincha, D.K., Heyer, A.G.: The role of raffinose in the cold acclimation response of Arabidopsis thaliana. FEBS Lett. 576, 169--173 (2004).
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BibTeX
@article{Zuther2004, abstract = {In many plants raffinose family oligosaccharides are accumulated during cold acclimation. The contribution of raffinose accumulation to freezing tolerance is not clear. Here, we investigated whether synthesis of raffinose is an essential component for acquiring frost tolerance. We created transgenic lines of Arabidopsis thaliana accessions Columbia-0 and Cape Verde Islands constitutively overexpressing a galactinol synthase (GS) gene from cucumber. GS overexpressing lines contained up to 20 times as much raffinose as the respective wild type under non-acclimated conditions and up to 2.3 times more after 14 days of cold acclimation at 4degreesC. Furthermore, we used a mutant carrying a knockout of the endogenous raffinose synthase (RS) gene. Raffinose was completely absent in this mutant. However, neither the freezing tolerance of non-acclimated leaves, nor their ability to cold acclimate were influenced in the RS mutant or in the G overexpressing lines. We conclude that raffinose is not essential for basic freezing tolerance or for cold acclimation of A. thaliana. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. [References: 30]}, author = {Zuther, E. and Büchel, K. and Hundertmark, M. and Stitt, M. and Hincha, D.K. and Heyer, A.G.}, comment = {DB - Current Contents PT - Article <9>}, journal = {FEBS Lett.}, month = {10}, number = {1-2}, owner = {heyer}, pages = {169--173}, privnote = {DB - Current Contents PT - Article <9>}, refid = {8}, title = {The role of raffinose in the cold acclimation response of Arabidopsis thaliana}, volume = 576, year = 2004 }
Rohde, P., Hincha, D.K., Heyer, A.G.: Heterosis in the freezing tolerance of crosses between two Arabidopsis thaliana accessions (Columbia-0 and C24) that show differences in non-acclimated and acclimated freezing tolerance. The Plant Journal. 38, 790–799 (2004). https://doi.org/10.1111/j.1365-313X.2004.02080.x.
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@article{rohde2004, abstract = {Summary Heterosis is broadly defined as the increased vigour of hybrids in comparison to their parents. In the model plant Arabidopsis thaliana, a significant heterosis effect on leaf-freezing tolerance was observed in the F1 generation of a cross between the accessions Columbia-0 (Col) and C24. Parental Col plants were significantly more freezing-tolerant than C24 plants in both the acclimated and non-acclimated (NA) states. Mid-parent heterosis was observed in the F1 plants, both in the basic tolerance of non-adapted plants and in freezing tolerance after cold acclimation. Best-parent heterosis, on the other hand, was only found after cold acclimation. The heterosis effect was reduced in the F2 populations such that only mid-parent heterosis was evident. The leaf content of soluble sugars (fructose (Fru), glucose (Glc), sucrose (Suc) and raffinose (Raf)) increased dramatically in the F1 plants after cold acclimation as compared to the parental lines. The content of proline (Pro), however, was only moderately increased in the F1 plants under the same conditions. Correlation analyses showed that only Raf content was consistently related to leaf-freezing tolerance in both the acclimated and NA states. A quantification of mRNA levels in leaves of parental and F1 lines using quantitative real-time RT-PCR showed no clear indication for an involvement of the investigated genes (CBF (C-repeat binding factor)1, CBF2, (cold-regulated protein (COR) 6.6, COR15a, COR15b, COR47 and COR78) in the heterosis effect. }, author = {Rohde, Peter and Hincha, Dirk K. and Heyer, Arnd G.}, doi = {10.1111/j.1365-313X.2004.02080.x}, journal = {The Plant Journal}, pages = {790-799}, series = 5, title = {Heterosis in the freezing tolerance of crosses between two Arabidopsis thaliana accessions (Columbia-0 and C24) that show differences in non-acclimated and acclimated freezing tolerance}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-313X.2004.02080.x}, volume = 38, year = 2004 }
Link
https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-313X.2004.02080.x
Hincha, D.K., Zuther, E., Heyer, A.G.: The preservation of liposomes by raffinose family oligosaccharides during drying is mediated by effects on fusion and lipid phase transitions. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1612, 172--177 (2003). https://doi.org/10.1016/s0005-2736(03)00116-0.
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@article{Hincha_2003, author = {Hincha, Dirk K. and Zuther, Ellen and Heyer, Arnd G.}, doi = {10.1016/s0005-2736(03)00116-0}, journal = {Biochimica et Biophysica Acta ({BBA}) - Biomembranes}, month = {06}, number = 2, pages = {172--177}, publisher = {Elsevier {BV}}, title = {The preservation of liposomes by raffinose family oligosaccharides during drying is mediated by effects on fusion and lipid phase transitions}, url = {https://doi.org/10.1016%2Fs0005-2736%2803%2900116-0}, volume = 1612, year = 2003 }
Link
https://doi.org/10.1016%2Fs0005-2736%2803%2900116-0
Hincha, D.K., Zuther, E., Hellwege, E.M., Heyer, A.G.: Specific effects of fructo- and gluco-oligosaccharides in the preservation of liposomes during drying. Glycobiology. 12, 103--110 (2002). https://doi.org/10.1093/glycob/12.2.103.
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@article{hincha2002, abstract = {The fructan family of oligo- and polysaccharides is a group of molecules that have long been implicated as protective agents in the drought and freezing tolerance of many plant species. However, it has been unclear whether fructans have properties that make them better protectants for cellular structures than other sugars. We compared the effects of fructans and glucans on membrane stability during air-drying. Although glucans of increasing chain length were progressively less able to stabilize liposomes against leakage of aqueous content after rehydration, fructans showed increased protection. On the other hand, glucans became more effective in protecting liposomes against membrane fusion with increasing chain length, whereas fructans became less effective. Fourier transform infrared spectroscopy showed a reduction of the gel to liquid-crystalline phase transition temperature (Tm) of air-dried liposomes by approximately 25°C in the presence of sucrose and maltose. For the respective pentasaccharides, the reduction of Tm of the lipids was 9°C lower for samples containing fructan than for those containing glucan, indicating increased sugar–membrane interactions for the fructan compared to the glucan. A reduced interaction of the longer-chain glucans and an increased interaction of the respective fructans with the phospholipid head groups in the dry state was also indicated by dramatic differences in the phosphate asymmetric stretch region of the infrared spectrum. Collectively, our data indicate that the fructo-oligosaccharides accumulated in many plant species under stress conditions could indeed play an important role in cellular dehydration tolerance.}, author = {Hincha, Dirk K. and Zuther, Ellen and Hellwege, Elke M. and Heyer, Arnd G.}, comment = {10.1093/glycob/12.2.103}, doi = {10.1093/glycob/12.2.103}, issn = {09596658}, journal = {Glycobiology}, month = {02}, number = 2, pages = {103--110}, privnote = {10.1093/glycob/12.2.103}, title = {Specific effects of fructo- and gluco-oligosaccharides in the preservation of liposomes during drying}, url = {http://dx.doi.org/10.1093/glycob/12.2.103ER}, volume = 12, year = 2002 }
Link
http://dx.doi.org/10.1093/glycob/12.2.103ER
Popova, A.V., Heyer, A.G., Hincha, D.K.: Differential destabilization of membranes by tryptophan and phenylalanine during freezing: the roles of lipid composition and membrane fusion. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1561, 109--118 (2002). https://doi.org/10.1016/s0005-2736(01)00462-x.
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@article{Popova_2002, author = {Popova, Antoaneta V. and Heyer, Arnd G. and Hincha, Dirk K.}, doi = {10.1016/s0005-2736(01)00462-x}, journal = {Biochimica et Biophysica Acta ({BBA}) - Biomembranes}, month = {03}, number = 1, pages = {109--118}, publisher = {Elsevier {BV}}, title = {Differential destabilization of membranes by tryptophan and phenylalanine during freezing: the roles of lipid composition and membrane fusion}, url = {https://doi.org/10.1016%2Fs0005-2736%2801%2900462-x}, volume = 1561, year = 2002 }
Link
https://doi.org/10.1016%2Fs0005-2736%2801%2900462-x
Oliver, A.E., Leprince, O., Wolkers, W.F., Hincha, D.K., Heyer, A.G., Crowe, J.H.: Non-Disaccharide-Based Mechanisms of Protection during Drying. Cryobiology. 43, 151--167 (2001). https://doi.org/10.1006/cryo.2001.2359.
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@article{Oliver_2001, author = {Oliver, Ann E. and Leprince, Olivier and Wolkers, Willem F. and Hincha, Dirk K. and Heyer, Arnd G. and Crowe, John H.}, doi = {10.1006/cryo.2001.2359}, journal = {Cryobiology}, month = {09}, number = 2, pages = {151--167}, publisher = {Elsevier {BV}}, title = {Non-Disaccharide-Based Mechanisms of Protection during Drying}, url = {https://doi.org/10.1006%2Fcryo.2001.2359}, volume = 43, year = 2001 }
Link
https://doi.org/10.1006%2Fcryo.2001.2359
Hincha, D.K., Hellwege, E.M., Heyer, A.G., Crowe, J.H.: Plant fructans stabilize phosphatidylcholine liposomes during freeze-drying. European Journal of Biochemistry. 267, 535–540 (2000). https://doi.org/10.1046/j.1432-1327.2000.01028.x.
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@article{hincha2000, abstract = {Fructans have been implicated as protective agents in the drought and freezing tolerance of many plant species. A direct proof of their ability to stabilize biological structures under stress conditions, however, is still lacking. Here we show that inulins (linear fructose polymers) isolated from chicory roots and dahlia tubers stabilize egg phosphatidylcholine large unilamellar vesicles during freeze-drying, while another polysaccharide, hydroxyethyl starch, was completely ineffective. Liposome stability was assessed after rehydration by measuring retention of the soluble fluorescent dye carboxyfluorescein and bilayer fusion. Inulin was an especially effective stabilizer in combination with glucose. Analysis by HPLC showed that the commercial inulin preparations used in our study contained no low molecular mass sugars that could be responsible for the observed stabilizing effect of the fructans. Fourier transform infrared spectroscopy showed a reduction of the gel to liquid-crystalline phase transition temperature of dry egg PtdCho by more than 20 °C in the presence of inulin. A direct interaction of inulin with the phospholipid in the dry state was also indicated by dramatic differences in the phosphate asymmetric stretch region of the infrared spectrum between samples with and without the polysaccharide. }, author = {Hincha, Dirk K. and Hellwege, Elke M. and Heyer, Arnd G. and Crowe, John H.}, doi = {10.1046/j.1432-1327.2000.01028.x}, journal = {European Journal of Biochemistry}, pages = {535-540}, series = 2, title = {Plant fructans stabilize phosphatidylcholine liposomes during freeze-drying}, url = {https://febs.onlinelibrary.wiley.com/doi/abs/10.1046/j.1432-1327.2000.01028.x}, volume = 267, year = 2000 }
Link
https://febs.onlinelibrary.wiley.com/doi/abs/10.1046/j.1432-1327.2000.01028.x

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