Bioanalytics II

Biophysics Group / Nussberger Lab

Modern bioanalytics contributes to the solution of challenging tasks in different areas of life science. Important tools include classical techniques such as x-ray crystallography and cryo-electron microscopy for the three-dimensional structure determination of large macromolecular complexes, super-resolution microscopy for the resolution of objects below the diffraction limit of 200 nm, and nano-pore arrays for high-resolution single-molecule analysis. This lecture series deals with these topics; it is designed for students in the master's programs in Technical Biology and Biomedical Engineering.

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C@MPUS

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ILIAS

General Information

Lecturer
Term

Winter

Credits

12 ETCS (including lab course), 6 ETCS (without lab course) 

Language

German or English

Schedule

Thursdays 9:45 - 11:15 h / PWR 57 V9.120

  • Starting with the winter semester 2022, all lectures are recorded and made available for download in ILIAS. Information on the dates will be available in CAMPUS

Main Topics

  • Topic 1: Fundamentals of X-ray crystallography 
    • 3D protein crystallization, crystal symmetries
    • Bravais lattices, Fourier theory, Patterson function
    • Ewald construction 
    • Solution of the phase problem
  • Topic 2: Basics of Cryo-Transmission Electron Microscopy
  • Topic 3: Super-resolution microscopy 
    • Near-field optical microscopy and total internal reflection fluorescence microscopy (TIRFM)
    • Stochastic Optical Reconstruction Microscopy (STORM) and Photoactivated Localization Microscopy (PALM)
    • Stimulated Emission Depletion Microscopy (STED)
  • Topic 4: Methods for manipulating individual molecules
    • Optical tweezers (Nussberger)
    • Scanning Probe Microscopy (AFM, SNOM)
  • Topic 5: Electrophysiological Methods
    • Patch Clamp
    • Single Molecule DNA Sequencing
    • Droplet hydrogel bilayer
  • An up-to-date overview with lecture dates can be found in CAMPUS.
  • All documents are available for download in the ILIAS course.
Literature
  • Bernhard Rupp, Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology (Garland, 2010)
  • Paul R. Selvin and Taekjip Ha, Single-Molecule Techniques (Cold Spring Harbor Laboratory Press 2008)
  • Igor N. Serdyuk, Nathan R. Zaccai and Jospeph Zaccai, Methods in Molecular Biophysics (Cambridge, 2007)
  • Lottspeich, Engels (Hrsg.) Bioanalytik (Springer Spektrum) 
  • Joseph R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, 2006) 
  • Seminar paper and lecture on a topic of molecular bioanalytics
  • Submission of the seminar paper and date of the lecture: End of the semester
  • Successful participation in the lab course 

Contact

 

Biophysics Group, Institute of Biomaterials and biomolecular Systems, University of Stuttgart

Pfaffenwaldring 57 , D-70569 Stuttgart, Germany

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