Bioanalytics I

Biophysics Group / Nussberger Lab

The qualitative and quantitative tracking of biological processes at the molecular level requires direct or indirect detection methods of macromolecules (proteins, nucleic acids) or low-molecular compounds (metabolites). Focussing on targets of different functional cellular levels has enormously increased the demands on multiple data collection. In this lecture, we deal with the basic (structural) analytical and preparative methods that are used and applied on a daily basis in almost every bioscience laboratory. The focus is on understanding the underlying methodological mechanisms and the resulting complexity of biological data sets. The aim is to encourage students to critically analyze both their own and published experimental results. This lecture is designed for students on the Bachelor's degree programme in Technical Biology.

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C@MPUS

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ILIAS

General Information

Lecturer
Term

Summer

Credits

6 ETCS

Language

German

Schedule

Thursdays 15:30 - 17:00 Uhr / Allmand. 31 0.106

  • The next lecture will take place next summer term.

Main topics

  • Topic 0: Introduction - Diversity of modern bioanalytics - Basic concepts and statistics (1 lecture) (Teleki)
  • Topic 1: Bioanalytics of macromolecules - Separation and detection techniques (3 lectures)
    • Protein analysis: Colourimetry (Biuret, Lowry, BCA, Bradford), spectroscopy A280/A205, fluorometry (Teleki)
    • Fundamentals of enzyme kinetics and activity tests (Teleki)
    • Electrophoretic methods and electroblotting: gel electrophoresis, Southern/Northern/Western blotting, capillary electrophoresis (Teleki)
  • Topic 2: Chromatographic techniques in quantitative bioanalysis (2 lectures)
    • Preparative FPLC chromatography: ion exchange, affinity chromatography, exclusion (Teleki)
    • Analytical HPLC and GC chromatography: Absorption (normal and reversed phase), distribution (HILIC) (Teleki)
  • Topic 3: Coupled (tandem) mass spectrometric methods (2 VL)
    • Protein analysis: MALDI-MS method (matrix-assisted laser desorption ionisation mass spectrometry) (Teleki)
    • ESI-MS(/MS) method for the quantification of low-molecular compounds (metabolomics) (Teleki)
  • Topic 3: Optical concentration and structure analysis (2 VL)
    • UV/VIS techniques in the bioanalysis of proteins and nucleic acids (Nussberger)
    • Circular dichrosimus and optical rotation dispersion for secondary structure analysis of proteins and chiral recognition of molecules (ORD, CD) (Nussberger)
    • Fluorescence-based bioanalysis (Nussberger)
    • Infrared spectroscopy (IR, FTIR, Raman) (Nussberger)
Informations
  • An up-to-date overview with lecture dates can be found at C @ MPUS
  • All documents are available for download in the ILIAS course.
Literature
  • Lottspeich, Engels (Hrsg.) Bioanalytik (Springer Spektrum) 
  • for reference, see C@MPUS
  • Oral exam (30 min)
  • Successful participation requires regular participation in the lecture.
  • In addition, regular participation in the lab course Bioanalytik I is expected.

Contact

 

Biophysics Group, Institute of Biomaterials and biomolecular Systems, University of Stuttgart

Pfaffenwaldring 57 , D-70569 Stuttgart, Germany

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